Amy R. Nielsen – Cannabis and Crohn’s

Amy R. Nielsen – DCNc | HealthySelf Nutrition LLC Feb 2 – Room 1: 3PM – Cannabis and Crohn’s Feb 3 – Room 1: 11AM – CBD and diabetes Feb 4 – Room 1: 11AM -. Cannabis and metabolic syndrome Amy Nielsen is a classically trained Chef and Herbalist pursuing a Doctoral degree in clinical nutrition, studying the development of culturally appropriate paradigms for nutrition management of metabolic syndrome, including diabetes, cardiovascular disease/hypertension, and lipedema/lymphedema. Amy has been working with clients for over 20 years, developing personalized plans to manage complex health concerns using diet, herbs, and lifestyle modification to meet client-centered goals. An avid researcher, Amy uses science-backed information and clinical experience to formulate theories and guiding principles for health management. They enjoy parsing complex medical information into actionable steps to initiate change and maintain sustainable solutions for diverse goals.

so welcome to the very first uh introductory um uh lecture here at theHawaii Cannabis Expo um my name is Amy Robertson neelsen I am a clinicaldoctoral clinical student um in clinical nutrition um I will be graduating with my doctoral degree in May I’m veryexcited about that um so we are uh today we’re going to talk about my favoritesubject which is Cannabis sativa and Crohn’s disease so Crohn’s disease is agastrointestinal it’s a stomach kind of stomach and and GI tract kind of condition and we’re going to talk aboutwhether or not whole plant cannabis is going to be um a potential for uh pathway for long-termremission so again my name is Amy neelsen as it says here I’m a doctoral candidate in clinical nutrition myuniversity is Maryland University of Integrative Health um I am also a uhtraditional Western herbalist I am a chef I’m a community health worker um and I work out in the uh Haula cooaarea um disclosures I have no Financial disclosures all this research is my ownthis is a narrative review a narrative review based on my clinical uh observations and my personal clinicalresources uh research um this is how we tell stories in science literature umnarrative reviews are usually a um a topic and then a um a discussion of whatis currently in literature for that for that topic so let’s Dive Right In what is thehistorical and anecdotal evidence for uh Cannabis sativa in abdominal and GItract issues um this is from the felter and Lloyd quote that we have up here isif you read the date on that 1898 so this is a very old quote um Ilike going back in history this plant has been around and uh working with us for more than 5,000 years it predateshuman uh humans um so felter and Lloyd way back in 1898 said it should beadministered in painful states of the stomach as in gastric neuralgia nervousgastralgia gastric ulcers where opium is inadmissible um and pain is due toindigestion so way back then they already knew that this plant uh wasuseful for these things patients OB often report that cannabis can relievethe symptoms including abdominal pain nausea diarrhea anorexia and Kia Kia isa is the inability to eat um it improves your mood it improves your Quality ofLife currently about 17 to 18% of 7even to 18% of IBD patients uh report usingcannabis to uh relieve their symptoms however only about a third of patientsactually report their use to their medical professionals that talks about the stigma that we still have in um inthe industry and uh in the in in the world for this plant however we do know that it’s been being used way back inthe 1800s and way back beyond that we know that this plant has been working with us for about 5,000 yearsnow so what is Crohn’s disease this is a picture of the inside of your GI tractof the inside of your small intestin um and it talks specifically about um transmural means that thisdisease goes all the way through all of the the tissue types that are in your GI tract it starts at the inside this iswhere your your food comes in right here and then this would be down to your blood cell your your blood system andthe rest of your system here so it goes all the way through as shown out righthere um there is a specific genetic component that goes along with this witha um with a comp um with a gene called the NO2 Gene it’s a pathway um that umuh talks about inflammation there is a derangement to the ability to distinguish the commensalversus pathogenic bacteria we have our microbiome that lives in our GI tractthis NO2 Gene um changes how we are able to uh um understand and assess the uhthe problem bacteria that are in our GI tract and those that are supposed to be there it upregulates the inflammatoryprocess it causes what’s called skip lesions so that means on the inside of the GI tract you will have areas thathave have uh that have inflammation area in areas thatdon’t those areas have this really very distinct cobblestone look that’s whatthey’re talking about here is this cobblestoning look here and then on the outside of yourintestine there’s this very specific Brown fat cap it’s a um calledmesenteric fat matted mesenteric adapost tissue and that’s the other distinguishing Mark uh distinguishingtissue for Crohn’s disease the way that it works the way that we develop Crohn’s disease thereare these positive agents things that cause Crohn’s disease we don’t know exactly how we develop Crohn’s diseasebut we think that it starts with some bacteria viruses and possibly dietary uh inflammation then we get some otherenvironmental inflammation uh environmental triggers and then we get some of some of our genetic pieces aswell that causes um these intestinal bacteria to get all um to change theirtheir communities um and change their digestive enzymes and the and the bileacids that you have in your system so those um those are depleted in thissystem some of the initiating triggers for Cron’s disease um toxicant inducedloss of Tolerance this is something um we were talking about this a little bit earlier just before we started thispresentation we live in environment uh our bodies are designed to live in an environment where we have 80% good stuffand 20% bad stuff that’s why we have a liver and that’s why we have kidneys to clear that bad stuff out of oursystem these days we live in a in a world where we have 80% bad stuff and20% good stuff right so that’s that toxicant loss of uh toxicant inducedloss of tolerance means that there are so many toxicant out there that we lose tolerance for everything so we don’thave the ability to clear anything so other initiating triggers for this number one is smoking numberone cause for for uh can for Crohn’s disease is smoking also lower plasmalevels of vitamin D we all live inside we put on lots of sunscreen even here inHawaii we have a large number of our population that has a very low um uhamount of vitamin D in their system increasing your vitamin D increases the ability for your GI tract to stayintact oral contraceptives can uh be an initiating trigger postmenopausal uhhormone Replacements so all those ladies on HRT this is another one aspirins andnids we already know that aspirin and IDs are really good at poking holes in your GI tract that causes inflammationthat causes that transmural that that all the way through the tissues that causes that kind of uh that kind oflesion in there antibiotics antibiotics shift the way that your um that yourmicrobiome works and that the the ratios of of uh bacteria commensal bacteria inyour microbiome and that can cause uh or initiate a Crohn’s flare or Crohn’sepisode depression and psychosis stress psychological stress we know that psychological stress increases uhinflammation in the body and no different with crohn’s disease and thendiet nod2 um Crohn’s disease is caused by aloss of regulatory capacity of the immune apparatus so what does that meanregulatory capacity how we regulate it is it fast is it slow is it absorbingand is not absorbing the immune apparatus is our immune system in our GItract N2 is a cellular intercellular bacterial sensor that means that itlives between your cells and it senses the bacteria between your cells and itsmutations are associated with the development of of CD of Crohn’s DiseaseIBD also is part of this um and is thought to be result from theinappropriate and continuing activation of this system of the mucos of that that lining of your GItract um and this is driven by the presence of yournormal um the normal commensal bacteriaso where does the the endocannabinoid system sit in terms and in terms of the rest of the systems in our body well theendocan system short ECS because it’s a really long word sits right here in the middle between your central nervoussystem your HPA axis that’s the hypothalamus pituitary adrenal axisthat’s where your stress uh is regulated that’s where your hormones is regulated and then your immune system over here sothe end endocannabinoid system sit sits in between those three and regulates the three of them it modulates the tone ofthose the three of them where do we find problems with the endocannabinoid system that may be umthat may be expressed in other places if you have low endocannabinoidtone in your central nervous system and your HPA access you might end up with cardiovascular functionissues if you have a low endocannabinoid tone between your immune system and HPAaccess you might end up with fat regulation lipid regulationconcerns and then between the immune system and the central nervous system you may end up with glucose regulationconcerns and that’s where type two diabetes comes in so lipid regulation is going to be obesity cardiovascularfunction and then glucose regulation what does the normalendocannabinoid look like in the human GI tract this is a really really great paper um if you’re interested in the GItract and the OC canabo system uh please take a look at this 2016 paper um it’sreally really good and goes into some very very specific detail about where in the GI tract the different cannaboid uhthe the different parts of the endocannabinoid system are um are maintained so in the GI epithelium thatmeans those are the cells that are right between that are that are the ones that are actually doing the absorbing thatare right there um touching the microbiome touching the food touching things like that that are coming throughthere’s a lot of CB1 there’s a lot of these are two um enzymes of uh uh synthesis so that meansthat they are making parts of the endocan system and then these two down here um Nate PDL and dagel those onesare um degrading so those ones are clearing the endocannabinoid system those are in your epithelium so thoseare in the cells of the the lining of your gut the enteric nervous system your neryour central sorry your GI tract has its own nervous system I’m sure you’ve heardthat the GI tract is your second your second brain so your gut is your second brain if you think about it we have agut reaction to something right you know it and that has that has a very specific feel in your body that’s exactly thisright here that’s where CB1 and CB2 are both very highly expressed in that internervous system so in that that’s remember when we if we go back to the other slide that’s this space right herethat’s right here between the central nervous system and your immune system that’s your anic nervous systemhere your blood vessels and your smooth muscle layers there’s a lot of CB1 CB2receptors down there as well as well as some of our enzymes of of synthesis and degradation down there so what happenedthat’s the normal side that’s what it’s supposed to normally look look like right when we get to inflammation forfor in your GI tract in the epithelium in those cells that are lining your your uh GI tract CB2 goes way up ininflammation we’re going to talk about why CB2 goes way up in inflammation you see that the magel andthe dagel those are the two um uh enzymes of synthesis so those make theendoc canins in your body those are also upregulated howeverNate PDL which is a degradation which is one of those um one of those enzymesthat clears your uh endocannabinoids that goes down so what does that say if CB2 goes up and the enzymes of synthesisgo up it means that it’s working on modulating that tone and if the um ifthe enzyme of degradation goes down it means that it’s holding those things that are helping re uh relieve theinflammation in the system longer because you can’t clear them as quickly when that’s load when that’sdown lumino amyes that’s a big F fancy science word lumino propria is the holethat the food goes down remember you’ve got a big tube through the middle of your body that that’s called the luminopropria that space inside there that the food goes down that’s called lumino propria immunocytes are immune cellsimmuno sites are cells so immune cells so in those immune cells that are livinginside your GI tract inside the tube that’s your GI tract they have umincreased CB2 again especially macrophases macras like to go and eat things um and then again the enzymes ofsynthesis which means that they are making more of those ocano to again bring that tone down bring thatinflammation down there are lots of herbs that workon this system there are lots of herbs that work very very well on this system system my favorite one believe it or notis very simple chamomile cannabis however has all sortsof its own U cannaboids we know that that the Entourage effect so cannabis has lots of different compounds in it weknow that cannaboids um uh THC CBD CBN CBG cbv all of those and then morebecause we don’t even know all the ones that are out there yet we’re still learning about all of them each of themhas a different action in there right so each of them does a different thing soif you look at reduces inflammation here look at all these these cannaboids thatreduce inflammation why do you think see why do you think cannabis is so great for reducing inflammation because it’sgot lots of different things that do it and they hit lots of different targets in the body so when we hear wellcannabis does everything yes it does for a very good reason that Entourage effectright for all of those those cannaboids in there so they have spe theseconstituent these cannaboids have very specific herbal actions in different places choosing the right combination ofcannaboids um is a very big Target for future research where is thatimportant your strainer your kovar right your kovar is your chemicalcompounds is the chemical signature of the plant that you’re growing right now each one of them as you know hasdifferent chemical different chemical signature that signature is very important in terms of when you are umwhen what you’re going to do with that so if you want to have one that’s going to be uh reducing inflammation maybe youwant to grow a strain that has as many cannaboids in it as possible but if you’re going to do something like youwant to stimulate appetite maybe you want to focus on one that’s got high THCremember there is significant variation in the plant between plants betweenclones grown by different people and then when you get that into a human being every human being has a differentendocannabinoid tone and different um um and different ways that your body isgoing to use those cannaboids cannaboids are lipid and umethanol soluble which means that they are fat soluble and they are alcohol soluble um they are not water solublecannaboids are not water soluble there are lots of things that you can that you can pull out ofcannabis that is water soluble as in tea but cannaboids are not one ofthem so let’s talk about how these specific can I apologize for the next few slides they are very wordy um sothere’s a lot of information on here I did this so that if you want the slides um please at the end feel free to emailme come back to my booth I’m at 106 I can send you the slides today um if youwant any of this information so CBG so CBG canaba Geral um inhibits Mera filmbi biofilms so Mera it’s a really really great big infection that lives in yourskin and that lives by creating these biofilms it breaks up those biofilms it eradicates persistent up to50% of uh persistent cells in a dose of dependent manner in as low little as 30minutes that’s pretty amazing for MSA pretty amazing for MRSA has superantibacterial properties as well CBG is also antiproliferative which means itmakes cells not uh replicate as fast that might be helpful in cancer umantibacterial we know that that’s going to be good again for Mera and a few other things um andanti-glaucoma right so anti glaucoma so it helps reduce the glaucoma in youreyes CBD my favorite I love CBD it’s my favorite my favorite cannaboid uminhibits inflammation inflammation inflammation inflammation inflammation is Runaway inflammation is the basis forall diseases so CBD really inhibits inflammation and we know now a lot ofdifferent ways if you come to my talk on uh uh CBD and type 2 diabetes we’regoing to talk a lot about he how CBD inhibits inflammation CBD has a potent anti-emetic effectanti-emetic is anti- nausea so this is part of the reason why this uh this kind ofon works reallyreally well in um for those folks that are going through cancer treatment andare going through uh chemotherapy and have a lot of nausea so CBD is really great for that um it okay a lot of s a lot morescience here um blocks the activation of NPH oxidase associated withum with r is reactive oxygen species formation and tnf Alpha is a is aninflammatory um uh mediator in the body um reactive oxygen species so we knowthat oxygen causes damage so oxygen when you put a nail out then oxygen likeanything that that uh any metal oxygen causes it to rust does the same thingfor cells in your body it doesn’t make them rest but it helps degrade them so this here helps block the formation ofthose Rea o reactive oxygen species that’sCBD cbdv cannabid Varin uh cannabid oh I can’t pronounce it rightnow but it’s got the Varin on the end of it cbdv is really really really interesting um CBD and THC um and as weknow now through a lot of chemistry we now see a lot of CBD that’s THC we seeDelta 8 THC right so that is that’s a chemical isomer U which means it’s it’sa slightly changed version of CBD that turns into THC in this particular casecbdv turns into thcv under acidic conditions and bothsignificantly inhibit nausea so it may not be the CBD it may be the the cbdvthat’s that’s inhibiting the nausea in a big entourage uh that comes out of a plant you’re not sureabout thcv um has anti-inflammatory action atCB2 in mice we’re going to talk about mice in a minute it reduces edema andand thermal hypo hypo algesia which means that it it it uh reduces edema andit reduces pain uh pain Sensation from heat specifically it also suppresses foodintake and weight gain at CB1 the problem with that is that italso induces psychosis at the same time through cv1 we’ve done a lot of research onusing the CB1 receptor specifically for uh food intake and weight gain uh orweight loss um in the early 90 mid90sthere was sorry not mid 90s uh early 2000s that’s when it was because this was uh uh found in mid90s but early2000s um they did a lot of work uh researchers did a lot of work um on CB1thinking that we were going to we’re gonna we’re going to stuff up all those CB1 receptors and we’re going to make you lose a whole lot of weight well wemade you lose a whole lot of weight but we also induced psychosis at the same time um and that’s because thesereceptors are throughout the body they’re not only in the lower GI tract they’re not only in in the lower half ofyour body but they’re in your brain as well so if you block it in your in your lower half of your body it will gothrough the blood brain barrier and it will change the uh change the way it affects yourbrain THC we love THC it’s the one that makes us all happy and stuff however THCis able to reverse and suppress micras AC activation this is good and bad so micromacroasia are the uh the little cells that like to go eat things they’re the the munchy guys like this um when youreverse and suppress those that may be a good thing if they’re running away with your system but if they’re not then youmay not want to you may not want to um uh suppress those because you really want to make sure that those macras arestill doing their job rightthca we have our acid forms anybody remember the difference between between THC and thca THC is decarbox right sothca is the acid form that’s that comes from uh plants that have not been heatedum if you heat THC you bang off that acid and then you getTHC thca through one very specific uh receptor calledgpr55 um is super anti-inflammatory so may not want toburn all of your cannabis maybe you want to make a tincture or um or a sublingualthat’s out of raw or cured cannabis where you’re using the thca and that’sgoing to help you as a as an anti-inflammatory modulator as well thcp I can guarantee very fewpeople in this room have heard about thcp so thcp is the perfect fit for the CB1receptor there is very little of this in most cannabis strains very very littleof it because it has the highest affinity for CB1 and it get it inducesthe highest psychological or intoxicating effect um and that’s thcp however um thcp um also has a 35 33to 63% um um higher affinity for those things thanthe thca or the thcv that we talked about before so that means that it’sgoing to do those things that anti-inflammatory thing that thca does it’s going to do it even better up to 33to 63% time percent better tiny little bits in your in yourte in your in your plant though tiny tiny little bitstpes so we’ve talked about all the cannaboids or at least a few of the cannaboids in and how they’re going tomodulate the inflammation and how they’re going to modulate some of the other F factors in Crohn’s disease inyour GI tract specifically lowering the inflammation and modulating how uh theum how the cells um uh connect together in your GI tracttpin are the most studied of any of the constituents pretty much of any plantmedicine are the tpin um this paper if you’ve ever uh read Dr Russo’s 2011paper down here this diagram is directly from that paper if you have not read this paper if you’ve not read anythingby Dr Russo Stop open up your phone look up Dr Russo cannabis and read everythinghe’s written he also has a ton of really amazing um um interviews and things likethat out there that you can listen to instead of having to read his papers uh where he talks a lot about this but heis super super interested in tpin in particular so Turpin are bioactivemolecules that are responsible for the smell of essential oils um they are themost bioactive compounds in any plant so bioactive means that it’s going to workon your body right bioactive they are water soluble notlipid soluble not fat soluble and they are very volatile all rightum remember we said that cannaboids are not water soluble these guys are watersoluble some of them how do tpes affect uh motility inCrohn’s flares motility means uh how fast your GI tract is processing thingsdiarrhea that’s that’s motility right constipation that’s motility right umI’m a nutritionist I talk a lot of poop okay it’s just what I do um especially working with crohn’s and other other GIdisorders um how however uh this is a really really great um uh little uhdiagram here about some of our um most common of our tpin out here um thedifficulty with using Tarpin therapeutically is that they’re so volatile you open it up you know ifyou’ve opened a container of a little essential oil of peppermint I could put an essential oil peppermint out there inthe back corner of this room and within 15 minutes I’ll be able to smell it up here those B those molecules move aroundvery very quickly so they’re very hard to sort of to to to get into medicineshowever they are super super um helpful in terms of motility so these are onesthat we find in cannabis a lot merine uh we find mercine in uh mercine is sort ofa sort of a Musky peppery kind of uh smell or taste pining smells like pinetrees yes it does pH is not one that we talk an awful lot about um it’s um ithas sort of another musky sort of sort of smell to it um terpinolene and Alphatpol um tpol those two are um sort of spicy and um they are also in cloves andin cinnamon um lemon uh lemon here lemonslimes that’s where that one comes from linol is in lavender as well and betacaropine beta caropine is the one that’s in the vast majority of plants out thereum that have any kind of smell to it beta cophine is um in most of when youwhen you have an indicia that’s really really um sleepy then that’s what that’swhat you’re smelling um I’m going to go through these a little bit quickly I’m not goingto read through each of these ones um specifically but know that alphap pinene is anti-inflammatory it’s abronchodilator it’s a memory aid alphap pinene in Rosemary Rosemary for remembrance that’s what you’re thinkingabout here it’s a potent antibiotic lemon is a precursor to every othertpine so lemon can turn into lots of other things too um it’s very re readilymetabolized it’s also possibly stored in your adapost tissue just like your cannabinoids are stored in your adaposttissue and your fat tissues um it’s anti anti um uhanti anotic sorry and immune producing it’s anantioxidant beta caropine is a full Agonist to CB2 so this guy down herebeta caropine is a full Agonist to CB2 that means it attaches to the CB2 receptor really reallywell super anti-inflammatory Kylene oxide is adifferent compound than beta aene but it works very similarly um it’s an antifeedant for um for insects on Plantsbut it does similar things to your gut so it’s antifungal um and anti platlet aggregation so if you have um uhcardiovascular disease this might be one that you’re looking for mercine we talked about reducing um inflammationit’s also a muscle relaxant and it’s also analgesic which means that it deals with pain linol think about lavenderwhere do we see lavender we see Lavender in all of our sleep aids and that’s exactly what it does it’s super sedatingit’s also uh helps with pain anti-cep anti-convulsant um anti anti- anticwhich means it’s a pain and then it’s sedating uh neural um is an interestingone it’s also very sedating it smells um must musty like like uh like basementmusty so if you have if you’ve got that if You’ got a cannabis a cannabis strain that you open it up and go oh thatsmells like my grandma’s basement that’s this one here that’s nnid um phyl is increases Gaba which means it’s going to slow your systemdown but it also prevents um vitamin A tetrogen um uh which means that it change ithelps uh vitamin A not switch formswhat are the problems in the current available literature there is plenty of evidence that’s discussing themetabolism and function of Cannabis sativa but there’s not an awful lot of objective evidence in in literaturesupporting specifically Crohn’s disease so we know a lot of things that it can do to help with crohn’s disease likeanti-inflammatory anti-emetic helps with nausea helps with motility but there areno studies or very few studies and we’ll show you them in a second um that talk about Crohn’s diseasespecifically population studies it’s super hard to do human trials right now they’re in in the we can’t even do themin the United States all the human trials are being done in Europe or in Israel still um and the safety profile thereare several adverse reactions noted for cannabis believe it or not we know that um certain strains um and certainamounts of THC can uh cause people can cause certain people to have umpsychosis psychotic episodes we also know that for some people um uh CBD anduh and THC can cause GI upset so there are a couple of other ways that that uhthat it may or may not be the right um uh substance for certain people thereare no deaths reported from Cannabis that’s because there are no CB receptorsin the brain stem which is what where you control your breathing and that’s where you get in trouble if if you uh ifyou have um the cells in your brain in your brain stem and controled breathing that’s when we get into problems withlike cocaine and things like that where where it changes your respiration it changes your breathing there are none ofthose in the in cannabis and it is generally well tolerated by mostpeople so these are this is the extent of all of the studies currently that we have with cannabis humans and Crohn’sdisease count them three that’s it right because we can’t do these work wecan’t do this work yet yet yet is the big Pro the big word there yet we wantto be able to do this research descheduling is the way to do that not reschedulingdescheduling why do we need to have human studies rather than Mouse studies well mice are great and we know a lotabout how cannabis Works in mice but we also know that particularly for Crohn’sdisease remember at the very beginning we talked about the NO2 genes and how those affect uh the the sensing of theright microbes in your GI tract and that’s what specifically broken in Crohn’s disease is this NO2 well inhumans that are homozygous means that we have two copies of this particular snipthis particular uh variant of the nood D2 Gene we me um develop Crohn’s diseaseum automatically mice doesn’t happen they have to induce it they have to give the mice stuff that makes their GI tractbad in order to give to make them have Crohn’s disease so we’re not using the same cells the same Pathways the waythat that they happen in the GI in the human GI tract the other problem is that in M mice their CB2 receptor functionsand looks very different whole different amino acid sequence whole differentreceptor sites sitting where it sits on the cells whole different places um that they sit in the in the body so that’sgoing to change how that system works so mice not the greatest analogfor humans in terms of Crohn’s Disease and CB uh and and cannabis um uhcannabis work specifically mice are cute we know a lot of things about mice just not an awful lot about Crohn’s and anduh and and cannabis yet questions any questions andconversations from the group out there I know I’m the first one it’s quiet yesgo ahead again part maybe this answer but with chirin right are you reallygetting the same chirine profile if you’re getting it in an edible or ateach versus the actual flower when it’s fresh no you can’t possibly be unlessthey are augmented to to to simulate the same the same strain because tpine areso incredibly volatile so if when and this is a good question when you extract from Cannabishow you extract my favorite teacher Dr Kevin Spelman says when you make an extractionfrom Cannabis you make a decision as to what you’re going to leave behind soevery time you choose whether you’re going to whether you’re going to use alcohol or you’re going to use glycerinor you’re going to smoke or anything like that you choose what you’re going to leave behind and that’s why your tinure ofACDC is going to work differently than an edible with ACDC then smoking ACDCthen even vaping ACDC so it’s going to be different because every time you makean extraction you choose what you’re going to leave behind and you’re only using those things that can be extracteduh in that in that particular process so with tpin it’s doubly uh that waybecause of the volatility of those compounds good question anything else any otherquestions so like if if you’re wanting a certain tpine profile then are you goingto want to maybe have the freshest flower to make sure you’re getting that specific Chine profile yes if you wantthe question was if you wanted the specific tarpine profile should you look for the freshest flower with that tpineprofile absolutely absolutely and maybe you want to find out what that tarpineprofile is go and have your have your cannabis tested and find out what the tpine profile is and augment that addessential oils right so you can smoke your cannabis and then have your diffusergoing with the same with the same cannaboid the same tarpine profile that’s going to augment what you alreadyare smoking in your in your uh in your pipe or wherever right so that’s one way of doing that stacking your Wellnessexactly stacking your Wellness exactly exactly using as many senses as possibleremember these receptors and these cells these are in every cell in yourbody when we become us at a cellular level we use all of our cells to do thatso we’re not just using the cells in in our bronchioles in our lungs when we’re smoking cannabis we’re using our nosewe’re using our mouth we’re using all of those pieces right we’re using our GItract as well because some of that’s going to go down there too right so if you can stack it if you can augment sothis is one that I’ve heard recently and I’m sure you guys have heard too um mangoes and cannabis right so mangoeshave natural cannaboids in them and if you take those if you make a uh ediblewith mangos and your cannabis you will have a different impact because they have their own cannaboids that are augmenting that system so that’s why inIndia they drink the mango lass with yep yep exactly cuz you’re stacking nowayurveda is the so in India you’re talking about in India ayurveda is the master of stacking ayurveda andtraditional Chinese traditional Chinese medicine TCM both of those systems have been around so much longer thanconventional Western medicine that they already understand all these pieces andthey’ve been doing this for so much longer and and stacking those things is absolutely what they do and noticethey’re also using diet right because diet is where you get a lot or cannaboidstoo all right any other questions sure one more so I’m I’m justcurious I know there’s lots of different reasons people will have a different impact right so for me for example Idon’t feel anything when I just justd and versus other people it will calmthem or you know like there’s an impact yep and can you explain a little bit about what makes sure sure that has todo with your endocannabinoid tone so your tone is is the number of receptorsof all the VAR various varieties of receptors in the can in your endocannabinoid system and you may havea lower tone for CBD than somebody else does so if you naturally have a lowertone for CBD you will not have as much of a reaction to that to the to the Lianto the thing that’s going to attach to those cells it also is going to depend on howyou ingested that so Edibles the least useful and theleast effective and the least um uh easy to to titrate so easy to to sort of dosefor yourself because it’s so dependent on what you ate what you drank whetheryou’ve got good bile function do you even have a gallbladder do you not so Edibles are really really uh the theleast the least um uh we call effic ious so it’s it’s got the the least profilefor safety profile and useful profile for us um the fastest one is oral bualso through your cheeks or through under your tongue sublingual or through your cheeks those are either glycerins oralcohols um there are some new nanot technologies that are that are bu thatare bual as well um but those are your most effective ways obviously the fastest way to get it in is through yourlungs um second is through your is through your your or bualspay any other questions person like find out theirendid tone no not yet not yet yet it’s the operative term we’re working on itwe’re working on it um you can you can you can test for your cannaboids you cantest for your anic canids aea 2 a there are blood tests to do that they’re notwell they’re very specialized test still it’s not like you can go to lab cor and say I want an endocannabinoid test andtest all my endocannabinoids it would be great we’re hoping that that’s going to be happening soon but it hasn’t happenedyet all right thank you so much I appreciate it everybody let me give youuh the last of my information so again here’s my information my contact information is down here on the bottom Iam over at Booth 106 so you’re welcome to come over and have a chat with me sit on my couch and have a chat with methanks so much for coming everybody I appreciate it